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Electrophoretic Separation of Proteins
Bulbul Chakavarti, Deb Chakavarti
Electrophoresis is used to separate complex mixtures of proteins (e.g., from cells, subcellular fractions, column fractions, or immunoprecipitates), to investigate subunit compositions, and to verify homogeneity of protein samples. It can also serve to purify proteins for use in further applications. In polyacrylamide gel electrophoresis, proteins migrate in response to an electrical field through pores in a polyacrylamide gel matrix; pore size decreases with increasing acrylamide concentration. The combination of pore size and protein charge, size, and shape determines the migration rate of the protein. In this unit, the standard Laemmli method is described for discontinuous gel electrophoresis under denaturing conditions, i.e., in the presence of sodium dodecyl sulfate (SDS).
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The need for routine pre-operative coagulation screening tests (prothrombin time PT/partial thromboplastin time PTT) for healthy children undergoing elective tonsillectomy and/or adenoidectomy

Toker Asaf, Haim Reuveni, Tikva Yermiahu, Alberto Leiberman, Gabriel Gurman, Avi Porat, Pnina Schlaeffer, Shvarts Shifra, Joseph Kapelushnik

International journal of pediatric otorhinolaryngology 2001, vol. 61, no3, pp. 217-222

In some medical centers, the routine pre-operative evaluation of healthy children undergoing elective tonsillectomy and/or adenoidectomy (T and A) includes coagulation screening tests (PT, prothrombin Time; PTT, partial thromboplastin time; and INR, international normalized ratio). In this retrospective study, we determined whether there is a positive correlation between prolonged PT/PTT/INR tests in healthy children, with no prior medical history of coagulation problems, and bleeding during surgery and/or bleeding in the month following surgery. We reviewed the records of 416 elective T and A surgeries performed at the Soroka University Medical Center in Beer-Sheva, Israel, over the course of 1999. One hundred and twenty-one (29.1%) patients had preoperative prolonged PT values but only four (3.3%) of these patients experienced light bleeding during surgery. Seven (5.8%) of the 121 patients with prolonged PT tests experienced bleeding episodes during the 1st month subsequent to the surgery. Of the 65 (15.6%) patients who had prolonged pre-operative INR values, only three (4.6%) experienced light bleeding during surgery. Two (3.1%) patients with prolonged INR values experienced light bleeding during the 1st month subsequent to surgery. Sixty-one (14.7%) patients had prolonged first preoperative PTT values, only five of whom (8.2%) experienced light bleeding during surgery. Two (3.3%) of the 61 with prolonged PTT values experienced light bleeding during the 1st month subsequent to surgery. We therefore concluded that pre-operative coagulation screening tests provide low sensitivity and low bleeding predictive value. As such, routine coagulation tests before T &A are not indicated unless a medical history of bleeding tendency is suspected.

Yağ Asidi Metabolizması..

Aşağıdaki linkte bazı bilgiler var:
http://opa1.faseb.org/pdf/protfold.pdf

Tez konum 'biyolojik nano gözenekler ile nükleobaz sentezi' biyolojik nano gözenek olarak alfa hemolysin kullanıyorum.Alfa hemolysin ve moleküler biyoloji konularında bilgi lazımmmm

# mrtckr : Tez konum 'biyolojik nano gözenekler ile nükleobaz sentezi' biyolojik nano gözenek olarak alfa hemolysin kullanıyorum.Alfa hemolysin ve moleküler biyoloji konularında bilgi lazımmmm

http://www.nanott.hacettepe.edu.tr/nanobulten/15/nanobulten15_lr.pdf